Composite

Part:BBa_K4345018

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-30)


Tet/lac promoter - ccdB fused to sfGFP

The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//cds/reporter/gfp
//cds/transcriptionalregulator/activator
Parameters
control
emission
excitation